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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or glass pipet. Rehydrate the pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, one or two slants and/or a plate.
5. Incubate all tubes and plate at 26oC for 10 to 21 days.
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Validation list no. 31. Int. J. Syst. Bacteriol. 39: 495-497, 1989.
Blackall LL, et al. Nocardia pinensis sp. nov., an actinomycete found in activated sludge foams in Australia. J. Gen. Microbiol. 135: 1547-1558, 1989.
Chun J, et al. A proposal to reclassify Nocardia pinensis Blackall et al. as Skermania piniformis gen. nov., comb. nov.. Int. J. Syst. Bacteriol. 47: 127-131, 1997. PubMed: 8995814