Sphingomonas suberifaciens (van Bruggen et al.) Yabuuchi et al.
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3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate broth tubes at 28oC with shaking for 4 days. Incubate solid medium at 28oC for the same period.
Colonies are circular, predominately pulvinate with some umbonate in appearance and compact, with entire margins. As culture ages, colonies become irregular and wrinkled with umbonate margins. Growth in broth medium is flocculent. Strain does not grow on Hugh and Leifsons Oxidative-Fermentative Medium, which contains peptone.
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van Bruggen AH, et al. Rhizomonas suberifaciens gen. nov., sp. nov., the causal agent of corky root of lettuce. Int. J. Syst. Bacteriol. 40: 175-188, 1990.
van Bruggen AH, et al. Corky root of lettuce caused by strains of a gram-negative bacterium from muck soils of Florida, New York, and Wisconsin. Appl. Environ. Microbiol. 55: 2635-2640, 1989.