Campylobacter mucosalis (Lawson et al.) Roop et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Open vial according to enclosed instructions.
3. Withdraw 0.5 ml from a single tube of #1540 broth and rehydrate the pellet.
4. Aseptically transfer this aliquot back into the broth tube. An agar slant and /or plate of medium #1645 and additional broth tubes may be inoculated with 0.1 ml each of this suspension.
5. Incubate the broth tubes, slants, and plates at 37oC under anaerobic conditions. You may use an .anaerobe jar with catalyst and gas generator pack or other suitable means of producing anaerobic conditions. Be sure caps are loosen to facilitate gas exchange.
6. Initially, 3 to 7 days of incubation are required before visible growth is evident. A slight turbidity is seen in the broth; and small, clear, circular, convex, entire, smooth colonies on agar. Cells are medium, curved to spiral, motile, Gram negative rods.
Storage at liquid nitrogen temperatures, with 10% sterile glycerol as the cryoprotectant, is recommended for long-term preservation.
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Lawson GH, et al. Some features of Campylobacter sputorum subsp. mucosalis subsp. nov., nom. rev. and their taxonnomic significance. Int. J. Syst. Bacteriol. 31: 385-391, 1981.