Streptococcus sanguinis White and Niven emend. Kilian et al.
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an agar slant and/or plate.
5. Incubate all tubes and plates at 37oC for 24 hours.
On #44 plates, colonies are small, entire, glistening, circular, smooth, and flat. It exhibits alpha hemolysis on sheep blood agar.
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