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Helicobacter muridarum Lee et al.

49282

Product category
Bacteria
Strain designation
ST1
Type strain
Yes
Isolation source
Ileum of adult rat
Product format
Frozen
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Handling information

Medium
Temperature
37°C
Atmosphere
Microaerophilic
Handling procedure
1. Open vial according to enclosed instructions.  Thaw and aseptically transfer the entire content of the vial to a tube of fresh #18 broth (5 to 6 ml).  Mix well.

2. To obtain a biphasic culture, add 0.4 ml of the suspension to a #260 slant.  Add remaining 0.1 ml of the suspension to a #260 plate and streak for isolation.

3. Incubate at 37oC under microaerophilic conditions.  This organism requires additional free hydrogen for best growth.  To obtain this, use an anaerobe jar WITHOUT an active catalyst and with an ANAEROBIC gas generator pack, or other acceptable method, to obtain the desired gas mixture.  Incubate slant with cap loose.

4.       Within 3‑5 days of incubation, good growth should be obtained in the broth pool at the bottom of the slant.  Additional incubation may be required for colonies to appear on the plate.  Further subcultures can be made using broth pool as the inoculum source.  Subcultures will require only 24 to 48 hours of incubation.

Handling notes
This is a slow growing organism that requires moist conditions for best growth.  Growth at the broth/agar interface of the biphasic slant should occur within three days, but little turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy.  Organisms are thick short, curved, Gram negative motile rods with 2 to 3 spiral turns.  The presence of spheroid cells indicates that viability is being lost either due to age or too much exposure to oxygen.

Growth on agar takes longer than with the biphasic culture.  Colonies are gray or clear forming a spreading film over the surface of the agar.  Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods.  Viability also decreases with repeated subculturing. The cells do not Gram stain well using traditional procedures. To obtain the best results, use a basic fuchsin counterstain in place of the safranin.

          Once good growth is obtained, transfer or freeze the culture.  Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or ultra-low temperature freezer is recommended.

          Additional information on this culture is available on the ATCC web site at www.atcc.org.

History

Deposited as
Helicobacter muridarum Lee et al.
Depositors
A Lee
Type of isolate
Animal
Cross references
GenBank M80205 Helicobacter muridarum 16S ribosomal RNA.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Lee A, et al. Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int. J. Syst. Bacteriol. 42: 27-36, 1992. PubMed: 1736969

Mendes EN, et al. Helicobacter trogontum sp. nov., isolated from the rat intestine. Int. J. Syst. Bacteriol. 46: 916-921, 1996. PubMed: 8863417

type strain

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