Pseudomonas aeruginosa (Schroeter) Migula
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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Woolfrey BF, et al. Broth microdilution testing of Pseudomonas aeruginosa and aminoglycosides: need for employing dilutions differing by small arithmetic increments. J. Clin. Microbiol. 16: 663-667, 1982. PubMed: 6818244
Woolfrey BF, et al. Comparative evaluation of the micro-media system, sceptor, and MIC-2000 microdilution methods for testing Pseudomonas aeruginosa against gentamicin, tobramycin, and amikacin. J. Clin. Microbiol. 17: 312-316, 1983. PubMed: 6403574
Woolfrey BF, et al. Evaluation of the automicrobic system for susceptibility testing of Pseudomonas aeruginosa to gentamicin, tobramycin, and amikacin. J. Clin. Microbiol. 19: 502-505, 1984. PubMed: 6425360