Helicobacter felis Paster et al.
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This is a slowing organism which requires moist conditions for best growth. Growth at the broth/agar interface of the biphasic slant should occur within 3 days, but little turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy. This organism is a large, motile, Gram negative bacillus, with spiral ridges.
Growth on agar takes longer than the biphasic culture. Colonies are flat with some spreading. It is essential to use fresh, moist plates. The cells do not Gram stain well using traditional procedures. For best results, use a basic fuchsin counterstain in place of the safranin.
Once good growth in obtained, transfer or freeze the culture. Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or "ultra-low temperature" freezer is recommended.
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Paster BJ, et al. Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int. J. Syst. Bacteriol. 41: 31-38, 1991. PubMed: 1704791
Lee A, et al. Isolation of a spiral-shaped bacterium from the cat stomach. Infect. Immun. 56: 2843-2850, 1988. PubMed: 3169989
Melchers K, et al. Cloning and membrane topology of a P type ATPase from Helicobacter pylori. J. Biol. Chem. 271: 446-457, 1996. PubMed: 8550601
Mendes EN, et al. Helicobacter trogontum sp. nov., isolated from the rat intestine. Int. J. Syst. Bacteriol. 46: 916-921, 1996. PubMed: 8863417