Kurthia sibirica Belikova et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 26oC for 24 hours.
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Validation list no. 25. Int. J. Syst. Bacteriol. 38: 220-222, 1988.
Belikova VA, et al. Kurthia sibirica, a new bacterial species of the Kurthia genus. Mikrobiologiya 55: 831-835, 1986.