Xanthomonas alfalfae subsp. citrumelonis Schaad et al.
2. From a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Gabriel DW, et al. Reinstatement of Xanthomonas citri (ex Hasse) and X. phaseoli (ex Smith) to species and reclassification of all X. campestris pv. citri strains. Int. J. Syst. Bacteriol. 39: 14-22, 1989.