Mycobacterium cookii Kazda et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or
1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant of #173 agar.
5. After growth has been achieved, additional transfers may be made.
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Kazda J, et al. Mycobacterium cookii sp. nov.. Int. J. Syst. Bacteriol. 40: 217-223, 1990. PubMed: 1697763