Microbacterium aurantiacum Takeuchi and Hatano
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #44 agar slant and/or plate.
5. Incubate the tubes and plate at 37oC for 24 hours.
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Takeuchi M, Hatano K. Proposal of six new species in the genus Microbacterium and transfer of Flavobacterium marinotypicum ZoBell and Upham to the genus Microbacterium as Microbacterium maritypicum comb. nov.. Int. J. Syst. Bacteriol. 48: 973-982, 1998. PubMed: 9734054