Salimicrobium halophilum (Ventosa et al.) Yoon et al.
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2. From a single tube of #1660 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
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Validation list no. 32. Int. J. Syst. Bacteriol. 40: 105-106, 1990.
Ventosa A, et al. Bacillus halophilus sp.nov., a moderately halophilic Bacillus species. Syst. Appl. Microbiol. 12: 162-166, 1989.