Gluconacetobacter diazotrophicus (Gillis et al.) Yamada et al.
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2. Using a single tube of #1 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 48-72 hours.
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Gillis M, et al. Acetobacter diazotrophicus sp. nov., a nitrogen-fixing acetic acid bacterium associated with sugarcane. Int. J. Syst. Bacteriol. 39: 361-364, 1989.