Bacillus benzoevorans Pichinoty et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 48-72 hours.
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Pichinoty F, Asselineau J. Morphologie et cytologie de Bacillus benzoevorans, une nouvelle espece filamenteuse, engainee et mesophile, degradant. Ann. Microbiol. 135B: 199-207, 1984.
Pichinoty F, et al. Characterisation biochimique de Bacillus benzoevorans sp. nov., une novelle espece filamenteuse, engainee et mesophile, degradant divers acides aromatiques et phenols. Ann. Microbiol. 135B: 209-217, 1984.