Lactococcus raffinolactis Schleifer et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
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Validation list no. 25. Int. J. Syst. Bacteriol. 38: 220-222, 1988.
Schleifer KH, et al. Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov.. Syst. Appl. Microbiol. 6: 183-195, 1985.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.