Paenibacillus pabuli (Nakamura) Ash et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 30°C for 24 hours.
Vegetative cells are variably motile bacilli, occurring individually and in pairs.
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Nakamura LK. Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., nom. rev. Bacillus pabuli sp. nov., nom. rev., and Bacillus validus sp. nov., nom. rev.. Int. J. Syst. Bacteriol. 34: 224-226, 1984.
Validation list no. 52. Int. J. Syst. Bacteriol. 45: 197-198, 1995.
Ash C, et al. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64: 253-260, 1993. PubMed: 8085788