Paenibacillus lautus (Nakamura) Heyndrickx et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate all tubes and plate at 30°C for 24 to 48 hours.
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Nakamura LK. Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., nom. rev. Bacillus pabuli sp. nov., nom. rev., and Bacillus validus sp. nov., nom. rev.. Int. J. Syst. Bacteriol. 34: 224-226, 1984.
Heyndrickx M, et al. A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended descriptions of P. lautus and of P. peoriae. Int. J. Syst. Bacteriol. 46: 988-1003, 1996. PubMed: 8863428