Burkholderia plantarii (Azegami et al.) Urakami et al.
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2. From a single tube of #1794 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
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Azegami K, et al. Effect of iron limitation on "Pseudomonas plantarii" growth and tropolone and protein production. Appl. Environ. Microbiol. 54: 844-847, 1988.
Ann. Phytopathol. Soc. Jpn. 51: 315-317, 1985.
Azegami K, et al. Pseudomonas plantarii sp. nov., the causal agent of rice seedling blight. Int. J. Syst. Bacteriol. 37: 144-152, 1987.
Urakami T, et al. Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and description of Burkholderia vandii sp. nov.. Int. J. Syst. Bacteriol. 44: 235-245, 1994.