Azospirillum halopraeferens Reinhold et al.
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2. Using a single tube of #1614 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1614 agar slant and/or plate.
5. Incubate the tubes and plate at 41°C for 72 hours.
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Reinhold B, et al. Azospirillum halopraeferens sp. nov., a nitrogen-fixing organism associated with roots of kallar grass (Leptochloa fusca (L.)Kunth). Int. J. Syst. Bacteriol. 37: 43-51, 1987.