Acidomonas methanolica (Uhlig et al.) Urakami et al.
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2. Using a single tube of #1562 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1562 agar slant and/or plate.
5. Incubate the tubes and plate at 30oC for 48 hours. Additional incubation may be necessary for growth on solid media.
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Grundig MW, Babel W. The linear oxidation of formaldehyde to CO2 as the proper energy generating sequence for the assimilation of methanol in Acetobacter methanolicus MB58. Arch. Microbiol. 149: 149-155, 1987.
Urakami T, et al. Acidomonas gen. nov., incorporating Acetobacter methanolicus as Acidomonas methanolica comb. nov.. Int. J. Syst. Bacteriol. 39: 50-55, 1989.
Uhlig H, et al. Acetobacter methanolicus sp. nov., an acidophilic facultatively methylotrophic bacterium. Int. J. Syst. Bacteriol. 36: 317-322, 1986.