Pseudomonas viridiflava (Burkholder) Dowson
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 28°C for 24 to 48 hours.
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Jones JB, et al. Pseudomonas viridiflava: Causal agent of bacterial leaf blight of tomato. Plant Dis. 68: 341-342, 1984.