Oligella urethralis (Lautrop et al.) Rossau et al.
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2. Using a single tube of # broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #814 agar slant and/or plate.
5. Incubate the tubes and plate at 34°C with 5% CO2 for 48 hours.
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Rossau R, et al. Oligella, a new genus including Oligella urethralis comb. nov. (Formerly Moraxella urethralis) and Oligella ureolytica sp. nov. (Formerly CDC Group IVe): relationship to Taylorella equigenitalis and related taxa. Int J Syst Bacteriol 37: 198-210, 1987.