Campylobacter coli (Doyle) Veron and Chatelain
2. Using a single tube of #1115 or #177 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 slant, and/or plate.
5. Incubate tubes and plate at 37oC, under microaerophilic conditions, for 48 to 72 hours. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method. Incubate slant with cap loose.
Fluid Thioglycollate tube may be incubated aerobically.
To observe cells, examine a wet mount of the broth under phase microscopy. The organism is a straight to slightly curved Gram negative rod with darting motility. Motility is best observed in young cultures.
Colonies on agar at 48 hours of incubation are circular, entire, low convex, and translucent. Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods.
The cells do not Gram stain well using traditional procedures. To obtain the best results, use a basic fuchsin counterstain in place of the safranin.
Storage at liquid nitrogen temperatures, with 10% sterile glycerol as the cryoprotectant, is recommended for long-term preservation.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
Penner JL, et al. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur. J. Clin. Microbiol. 2: 378-383, 1983. PubMed: 6628376
Penner JL, Hennessy JN. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J. Clin. Microbiol. 12: 732-737, 1980. PubMed: 6796598