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Campylobacter jejuni (Jones et al.) Veron and Chatelain

43474

Product category
Bacteria
Strain designation
MK100
Type strain
No
Isolation source
Feces
Geographical isolation
Canada
Product format
Freeze-dried
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Serotype
O:20

Handling information

Medium
Temperature
37°C
Atmosphere
Microaerophilic
Handling procedure
1. Open vial according to enclosed instructions.

2. Using a single tube of #1115 or #177 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the pellet.

3. Aseptically transfer this aliquot back into the broth tube.  Mix well.

4. Use several drops of the suspension to inoculate a #260 slant, and/or plate.

5. Or, to obtain a biphasic culture, add 0.5 ml of the suspension to a #260 agar slant (see notes).

6.       Incubate tubes and plate at 37oC, under microaerophilic conditions, for 48 to 72 hours.  Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method.  Incubate slant with cap loose.

Handling notes
Fluid Thioglycollate tube may be incubated aerobically.

 This is a slow growing organism that requires moist conditions for best growth.  A biphasic culture will give the most rapid growth.  Growth at the broth/agar interface of the biphasic slant should occur within two to three days, but little turbidity will be seen.  To observe growth, examine a wet mount of the broth under phase microscopy.  The organism is a curved to spiral Gram negative rod with darting motility .  Motility is best observed in young cultures. 

Growth on agar takes longer than with the biphasic culture.  Colonies are circular, entire, convex, and translucent.

Older colonies develop irregular edges and raised centers.    Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods. 

          The cells do not Gram stain well using traditional procedures.  To obtain the best results, use a basic fuchsin counterstain in place of the safranin.

Storage at liquid nitrogen temperatures, with 10% sterile glycerol as the cryoprotectant, is recommended for long-term preservation.

Additional information on this culture is available on the ATCC web site at www.atcc.org.

History

Deposited as
Campylobacter coli (Doyle) Veron and Chatelain
Depositors
JL Penner
Chain of custody
ATCC <-- JL Penner <-- M. Karmali
Type of isolate
Human

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Penner JL, et al. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur. J. Clin. Microbiol. 2: 378-383, 1983. PubMed: 6628376

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