Vibrio mediterranei Pujalte and Garay
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 36-48 hours.
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Pujalte MJ, Garay E. Proposal of Vibrio mediterranei sp. nov.: A new marine member of the genus Vibrio. Int. J. Syst. Bacteriol. 36: 278-281, 1986.