Vibrio mediterranei Pujalte and Garay
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 slant, and/or plate
5. Incubate the tubes and plate at the recommended temperature for 24 to 48 hours.
6. After growth has been achieved, additional transfers may be made or cells prepared for storage. Most Vibrio species will die off rapidly once the stationary phase is reached, even at refrigerator temperatures. To avoid frequent transfers, it is recommended that cells be frozen with a suitable cryoprotectant.
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Pujalte MJ, Garay E. Proposal of Vibrio mediterranei sp. nov.: A new marine member of the genus Vibrio. Int. J. Syst. Bacteriol. 36: 278-281, 1986.