Campylobacter mucosalis (Lawson et al.) Roop et al.
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2. Open vial according to enclosed instructions.
3. Under anaerobic conditions, withdraw 0.5 ml from a single tube of #1540 broth and rehydrate the pellet.
4. Aseptically transfer this aliquot back into the broth tube. Additional broth can be inoculated using 0.2 ml of this cell suspension per tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
5. Incubate the broth tubes, slants, and plates at 37oC under anaerobic conditions. You may use an anaerobe jar with catalyst and gas generator pack or other suitable means of producing anaerobic conditions. Be sure caps are loosen to facilitate gas exchange. Incubate aerobic blood plate
aerobically at 37oC.
6. Initially, 3 to 7 days of incubation are required before visible growth is evident. A slight turbidity is seen in the broth; and small, clear, circular, convex, entire, smooth colonies on the anaerobic blood agar plates. No growth should be seen on the aerobic plate.
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Lawson GH, et al. Some features of Campylobacter sputorum subsp. mucosalis subsp. nov., nom. rev. and their taxonnomic significance. Int. J. Syst. Bacteriol. 31: 385-391, 1981.