Lactobacillus aviarius subsp. araffinosus Fujisawa et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of the recommended broth from a single tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant of #416 may also be inoculated with 0.1 ml.
5. Within 48 hours, growth should be evident by turbidity in the broth. Subsequent transfers should grow in 24 to 48 hours.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw‑caps on test tubes in anaerobic chamber,
· Loose screw‑caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that. an anaerobic gas headspace is retained.
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Validation list no. 20. Int. J. Syst. Bacteriol. 36: 354-356, 1986.
Fujisawa T, et al. Lactobacillus aviarius sp.nov.: a new species isolated from the intestines of chickens. Syst. Appl. Microbiol. 5: 414-420, 1984.