Arcobacter butzleri (Kiehlbauch et al.) Vandamme et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. Open vial according to enclosed instructions or visit www.atcc.org for
2. Rehydrate the entire pellet with approximately 0.5 mL of #1115 broth.
3. Aseptically transfer the entire contents to a 5-6 mL tube of #1115 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
4. Use several drops of the primary broth tube to inoculate a #260 plate and/or
#260 agar slant.
5. Or, to obtain a biphasic culture, add several drops of the primary broth tube to a #260 agar slant. Best practice is to incubate these slants at an angle.
6. Incubate at 30° C under microaerophilic conditions for 24-48 hours. Use an
anaerobe jar with an active catalyst and a microaerophilic gas generator pack or
other acceptable method. All tubes and slants should be incubated with caps
Storage at liquid nitrogen temperatures, with 10% sterile glycerol as the cryoprotectant, is recommended for long-term preservation.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Neill SD, et al. Taxonomic position of Campylobacter cryaerophila sp. nov.. Int. J. Syst. Bacteriol. 35: 342-356, 1985.