Arcobacter cryaerophilus (Neill et al.) Vandamme et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #1115 or #177 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24-48 hours under microaerophilic conditions. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method. Loosen the screw caps during an incubator period.
Fluid Thioglycollate tube may be incubated aerobically.
Storage at liquid nitrogen temperature, with 10% sterile glycerol as the cryoprotectant, is recommended for long term preservation.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Neill SD, et al. Taxonomic position of Campylobacter cryaerophila sp. nov.. Int. J. Syst. Bacteriol. 35: 342-356, 1985.
Arcobacter. Washington, DC:American Public Health Association;APHA APHA2001-30.2, 2001