Corynebacterium diphtheriae (Kruse) Lehmann and Neumann
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 37°C for 24 to 48 hours.
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Groman N, et al. Detection and expression of DNA homologous to the tox gene in nontoxinogenic isolates of Corynebacterium diphtheriae. Infect. Immun. 42: 48-56, 1983. PubMed: 6311753
Cianciotto N, Groman N. A beta-related corynebacteriophage which lacks a tox allele but can acquire it by recombination with converting phage. Infect. Immun. 49: 32-35, 1985. PubMed: 3924830