Xanthobacter autotrophicus (Baumgarten et al.) Wiegel et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or glass pipette. Rehydrate the entire pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 30oC for 48 hours.
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Janssen DB, et al. Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10. Appl. Environ. Microbiol. 49: 673-677, 1985. PubMed: 3994371
Keuning S, et al. Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10. J. Bacteriol. 163: 635-639, 1985. PubMed: 4019411