Zygosaccharomyces rouxii (Boutroux) Yarrow
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate cultures at recommended temperature.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Watanabe Y, et al. Characterization of plasma membrane proton-ATPase from salt-tolerant yeast Zygosaccharomyces rouxii cells. Yeast 7: 599-606, 1991.
Watanabe Y, et al. Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. J. Biochem. 110: 237-240, 1991. PubMed: 1837019
Kiuchi K, et al. Isolation of halotolerant yeasts capable of assimilating linoleate in miso. Nippon Shokuhin Kogyo Gakkaishi 25: 508-514, 1978.
Gordon JL, Wolfe KH. Recent allopolyploid origin of Zygosaccharomyces rouxii strain ATCC 42981. Yeast 25: 449-456, 2008. PubMed: 18509846