Candida kefyr (Beijerinck) van Uden et Buckley
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25° C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate appropriate solid or liquid media (see atcc.org for a recommended medium and temperature).
6. Incubate cultures at appropriate temperature. Remaining suspension may be stored in refrigerator for possible reuse, although no survival time can be specified.
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Beijerinck MW. Die Lactase, em neues Enzym. Centralbl. Bakteriol. Parasitenkd. 6: 44-48, 1889.
Rapin JD, et al. Glycerol production by yeast fermentation of whey permeate. Enzyme Microb. Technol. 16: 143-150, 1994.