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pSW 11-1 [pSW11-1]

41027

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.8; EcoRI--3.1, 0.6; PstI/EcoRI--2.6, 0.6, 0.5.
Detects a TaqI polymorphism mapping to chromosome 6.
A full-length Ki-ras cDNA capable of transformation if given a promoter. The PstI site on the 3' side of the insert appears to be missing. The insert contains HinfI (3), TaqI (3), Sau96I (2) and EcoRI sites.
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.900000095367432

Insert information

Insert size (kb)
1.1000000000000001
Type of DNA
cDNA
Insert source
SW480 cell line DNA transferred into NIH 3T3 cells
Insert tissue
SW480 cell line DNA transferred into NIH 3T3 cells
Gene product
Kirsten rat sarcoma 2 viral (v-Ki-ras2) oncogene homolog( c-Kirsten-ras proto-oncogene, oncogene KRAS2, cellular c-Ki-ras2 proto-oncogene) [KRAS2]

Handling information

History

Depositors
RA Weinberg
Cross references
GenBank L00045
GenBank 164214

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Siracusa LD, et al. A molecular genetic linkage map of mouse chromosome 2. Genomics 6: 491-504, 1990. PubMed: 1970329

Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597

McCoy MS, et al. Human colon carcinoma Ki-ras2 oncogene and its corresponding proto-oncogene. Mol. Cell. Biol. 4: 1577-1582, 1984. PubMed: 6092920

Barbara Weiffenbach, personal communication

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