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pPM1

40172

Clone type
Vector
Applications
Molecular biology
Product format
Frozen
Currently Unavailable
This product is not currently available for purchase.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
vector permitting RNA synthesis in vitro

Characteristics

Comments
The unique EcoRI site in the vector occurs downstream of an insert into the SmaI site. Plasmid with insert can be linearized with EcoRI to generate a transcript.
This is a vector for in vitro transcription from a modified lambda PR promoter.
Distributed in aliquots of 187.5 ng (3.75 ul, 50 ng/ul).
This vector contains the cI857 temperature sensitive mutant gene of the lambda cI repressor. Temperature control can be used to repress, in vivo, transcription of potentially deleterious genes during culture in Escherichia coli.
The PR promoter in this vector has been modified to position the cleavage site of a unique SmaI site exactly at the initiation site of the PR promoter.
Transcription will be initiated at the first nucleotide of any 5'-purine-terminated DNA fragment fused to the SmaI site.
In some cases, significant yields of transcript initiated at the first nucleotide of an inserted 5'-pyrimidine-terminated DNA fragment can also be obtained using this vector.
This was constructed by inserting a 0.9 kb ClaI-BamHI fragment from pCQV2 (containing lambdaPR and cI857) into M13mp9 (AccI/BamHI). This was used to make PstI/SmaI fragment with the SmaI site adjacent to the PR initiation site and inserted into pUC9.
Mycoplasma contamination
Not detected
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Vector information

Construct size (kb)
3.599999904632568
Vector name
pPM1 (plasmid)
Type of vector
plasmid
Construction
pUC9, pCQV2
Enhancer
none
Insert detection
lacZ'
Markers
ampR
Promoters
lambda PR
Replicon
pMB1
Repressor gene
cI857
Terminator
none

Handling information

Temperature
30°C

History

Depositors
Lubrizol Genetics, Inc.
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
4,885,248

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Frequently Asked Questions

References

Curated Citations

Ahlquist P, Janda M. cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Mol. Cell. Biol. 4: 2876-2882, 1984. PubMed: 6549346

Ahlquist P, et al. Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proc. Natl. Acad. Sci. USA 81: 7066-7070, 1984.

Ahlquist PG. Transfer vector. US Patent 4,885,248 dated Dec 5 1989

. . Methods Enzymol. 118: 704-716, 1986.

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