pcDmouseIL-2 [MT-1]

39892 ^™

Organism: Mus musculus, mouse
Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Clone type: Clone
Applications: Molecular biology
Product format: Frozen

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

General

Specific applications: in another host, produces protein interleukin 2

Characteristics

Comments: Restriction digests of the clone give the following sizes (kb): HindIII--3.2, 0.94; PstI--2.9, 1.35; XhoI--3.0, 1.1.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.70; BglII--0.31; HindIII--0.38; PstI--0.04; PvuII--0.20; SacI--0.22; SphI--0.06.
Contains the complete coding sequence (nt 49-557).
Digestion with XhoI excises the insert plus flanking vector sequence.
Mycoplasma contamination: Not detected
Technical information: ATCC Product Experience does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Vector information

Construct size (kb): 3.799999952316284
Intact vector size: 3.000
Vector name: pcDV1
Type of vector: plasmid
Construction: pBR322, SV40
Host range: vertebrate cells
Cloning sites: SalI; ClaI; HindIII; EcoRI; KpnI
Enhancer: none
Markers: ampR
Promoters: none
Replicon: pMB1
Terminator: SV40 late

Insert information

Insert size (kb): 0.81599999999999995
Type of DNA: cDNA
Insert source: T-cell line LB2-1 (ATCC CRL-8629), Con A-induced
Insert strain: C57BL/6
Insert tissue: T-cell line LB2-1 (ATCC CRL-8629), Con A-induced
Insert information: DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Cross references: DNA Seq. Acc.: K02292
Genome: mouse
Chromosome: 3

3
Target gene: interleukin 2
Gene name: interleukin 2
Gene product: interleukin 2 [Il2]
Gene symbol: Il2
Contains complete coding sequence: Yes

Handling information

Host: Escherichia coli MC1061; 3943B
Medium: ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37°C
Handling notes: Restriction digests of the clone give the following sizes (kb): HindIII--3.2,
0.94; PstI--2.9, 1.35; XhoI--3.0, 1.1.
- ATCC staff

Contains the complete coding sequence (nt 49-557). Digestion with XhoI excises
the insert plus flanking vector sequence. The insert contains the following
restriction sites (approximate kb from the 5' end): BglI--0.70; BglII--0.31;
HindIII--0.38; PstI--0.04; PvuII--0.20; SacI--0.22; SphI--0.06.
- Proc. Natl. Acad. Sci. USA 82: 68-72, 1985

.patent

History

Depositors: DNAX Research Institute of Molecular and Cellular Biology, Inc., EP Ching, DNAX Research Institute of Molecular and Cellular Biology, Inc.
Patent depository: This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number: 4,798,789
Cross references: GenBank K02292

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Yokota T, et al. Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc. Natl. Acad. Sci. USA 82: 68-72, 1985. PubMed: 3918306

Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742

Lee FD, et al. cDNA clones coding for polypeptides exhibiting murine interleukin-2 activity. US Patent 4,798,789 dated Jan 17 1989

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