PPFZ-R4 Plasmid in Escherichia coli
39543 ™
Documentation
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Detailed product information
General
- Specific applications
-
produces protein prorennin A prochymosin
Characteristics
- Comments
-
pPFZ-R2 (ATCC 39544) and pPFZ-R4 (ATCC 39543) are the same except that the ATG initiation codon follows the ribosomal binding site of the trp leader peptide by 5 bp in pPFZ-R2 and by 11 bp in pPFZ-R4.The insert contains BamHI sites. A KpnI/SalI digest should give a probe of about 970 bp which contains the sequences encoding aa 83 through the TGA stop codon.pPFZ-R2 (ATCC 39544) shows significantly higher expression of the protein product than pPFZ-R4 (ATCC 39543).Constructed from two fragments of pCR101, a 235 bp BamHI/KpnI fragment (encoding aa 6-83) and a 4772 bp HindIII/KpnI fragment (encoding aa 83 through TGA stop codon, pMB1, ampR) ligated with a 370 bp HindIII/BamHI fragment of ptrpLI-R4-B48.The 370 bp HindIII/BamHI fragment of ptrpLI-R4-B48 contains trp promoter-operator, ribosome binding site, artificial ATG initiation codon, and chemically derived sequence encoding the first 5 amino acids of the protein.
- Mycoplasma contamination
- Not detected
- Technical information
-
ATCC Product Experience does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Vector information
- Vector name
- unknown
- Type of vector
- plasmid
- Host range
-
Escherichia coli
- Markers
-
ampR
Insert information
- Type of DNA
- cDNA
- Genome
- bovine
- Target gene
-
prorennin A
- Gene name
- prorennin A
- Gene product
- prorennin A(prochymosin)
- Contains complete coding sequence
- Unknown
Handling information
- Host
-
Escherichia coli HB101 (ATCC 33694)
- Medium
- Temperature
-
37°C
- Handling notes
-
Constructed from two fragments of pCR101, a 235 bp BamHI/KpnI fragment (encoding
aa 6-83) and a 4772 bp HindIII/KpnI fragment (encoding aa 83 through TGA stop
codon, pMB1, ampR) ligated with a 370 bp HindIII/BamHI fragment of
ptrpLI-R4-B48. The 370 bp HindIII/BamHI fragment of ptrpLI-R4-B48 contains trp
promoter-operator, ribosome binding site, artificial ATG initiation codon, and
chemically derived sequence encoding the first 5 amino acids of the protein.
pPFZ-R2 (ATCC 39544) and pPFZ-R4 (ATCC 39543) are the same except that the ATG
initiation codon follows the ribosomal binding site of the trp leader peptide by
5 bp in pPFZ-R2 and by 11 bp in pPFZ-R4. pPFZ-R2 (ATCC 39544) shows
significantly higher expression of the protein product than pPFZ-R4 (ATCC
39543). The insert contains BamHI sites. A KpnI/SalI digest should give a probe
of about 970 bp which contains the sequences encoding aa 83 through the TGA stop
codon.
- U.S. Pat. 4,935,370 dated June 19, 1990
.patent
History
- Depositors
- Pfizer, Inc., LH Huang, Pfizer, Inc.
- Patent depository
-
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
- Patent number
-
4,935,370
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
- Disclosures
-
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Franke AE. Expression plasmids for improved production of heterologous protein in bacteria. US Patent 4,935,370 dated Jun 19 1990
Nishimori K, et al. Expression of cloned calf prochymosin gene sequence in Escherichia coli. Gene 19: 337-344, 1982. PubMed: 6295885
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