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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. From a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate all tubes and plate at 26oC for 7 to 10 days.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Iwami M, et al. New antitumor antibiotics, FR-900405 and FR-900406. I. Taxonomy of the producing strain. J. Antibiot. 38: 835-839, 1985. PubMed: 4030498
Kiyoto S, et al. New antitumor antibiotics, FR-900405 and FR-900406. II. Production, isolation, characterization and antitumor activity. J. Antibiot. 38: 840-848, 1985. PubMed: 4030499
Nishikawa M, et al. Biologically active WS 6049 substances, a process for the production thereof and their pharmaceutical compositions. US Patent 4,578,271 dated Mar 25 1986