pYAC55 plasmid in E. coli
37521 ™
This is a yeast artificial chromosome (YAC) vector and Saccharomyces cerevisiae to Escherichia coli shuttle vector useful for constructing genomic libraries. It was constructed from pYAC2 by replacing the SmaI site in the SUP4 intron with the NotI site, and eliminating the SfiI and NotI sites flanking the SUP4 gene.
Cloning into the NotI site located in the SUP4 intron inactivates expression. The HIS3 sequence is removed when producing the telomers by BamHI digestion.