Campylobacter sputorum biovar sputorum On et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Fluid Thioglycollate tube may be incubated aerobically.
This is a slow growing organism that requires moist conditions for best growth. A biphasic culture will give the most rapid growth. Growth at the broth/agar interface of the biphasic slant should occur within two to three days, but little turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy. Motility is usually observed only in young cultures.
Growth on agar takes longer than with the biphasic culture. Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods.
The cells do not Gram stain well using traditional procedures. To obtain the best results, use a basic fuchsin counterstain in place of the safranin.
Storage at liquid nitrogen temperatures, with 10% sterile glycerol as the cryoprotectant, is recommended for long-term preservation.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Roop II RM, et al. Designation of the neotype strain for Campylobacter sputorum (Prevot) Veron and Chatelain 1973. Int. J. Syst. Bacteriol. 36: 348, 1986.
On SL, et al. Emended description of Campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans. Int. J. Syst. Bacteriol. 48: 195-206, 1998.