Xanthomonas pisi (Goto and Okabe) Vauterin et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C.
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Dye DW, et al. International standards for naming pathovars of phytopathogenic bacteria and a list of pathovar names and pathotype strains. Rev. Plant Pathol. 59: 153-168, 1980.
Vauterin L, et al. Reclassification of Xanthomonas. Int. J. Syst. Bacteriol. 45: 472-489, 1995.