Taylorella equigenitalis (Taylor et al.) Sugimoto et al.
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Colonies on #814 agar plates are very small, entire, glistening, circular, smooth, and low convex.
If a broth culture is desired, use Medium #814 formulation without the agar, or HTM (Haemophilus Test Broth). To make HTM, first a fresh hematin stock solution is prepared by dissolving 50 mg of hematin powder in 100 mL of 0.01N (0.01 mol/L) NaOH with heat and stirring until the powder is dissolved thoroughly. Thirty milliliters of the hematin stock solution is added to 1 L of Mueller-Hinton Broth with 5 g of yeast extract. After autoclaving and cooling, aseptically add 3 mL of a NAD (nicotinamide adenine dinucleotide) stock solution (50 mg of NAD dissolved in 10 mL of distilled water; filter sterilized).
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Sugimoto C, et al. Transfer of Haemophilus equigenitalis Taylor et al. 1978 to the genus Taylorella gen. nov. as Taylorella equigenitalis comb. nov.. Curr. Microbiol. 9: 155-162, 1983.
Taylor CE, et al. The causative organism of contagious equine metritis 1977: proposal for a new species to be known as Haemophilus equigenitalis. Equine Vet. J. 10: 136-144, 1978. PubMed: 99302
Validation list no. 10. Int. J. Syst. Bacteriol. 33: 438-440, 1983.
Validation list no. 16. Int. J. Syst. Bacteriol. 34: 503-504, 1984.