Lactobacillus kefiri Kandler and Kunath
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #416 agar slant and/or plate.
5. Incubate tubes and plate at 35oC in an atmosphere of 5% CO2 for 24-48 hours. Loosen screw caps of all test tubes during the incubation period.
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Validation list no. 11. Int. J. Syst. Bacteriol. 33: 672-674, 1983.
Kandler O, Kunath P. Lactobacillus kefir sp. nov., a component of the microflora of kefir. Syst. Appl. Microbiol. 4: 286-294, 1983.
Patel RN, et al. Microbial reduction of 1-(4-fluorophenyl)-4-[4-(5-fluoro-2-pyrimidinyl)-1-piperazinyl]butan-1-one. Biotechnol. Appl. Biochem. 17: 139-153, 1993. PubMed: 8166884
Truper HG, De Clari L. Taxonomic note: Necessary correction of specific epithets formed as substantives (nouns) "in apposition". Int. J. Syst. Bacteriol. 47: 908-909, 1997.