Legionella santicrucis Brenner et al.
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2. From a single tube of #1099 broth (5 to 6 ml), withdraw approximately 0.5 to 1 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an agar slant and/or plate.
5. Incubate at 37°C for 48 to 72 hours.
The preferred medium for growth is CYE, in which strain is fluorescent. No growth will occur on sheep blood agar.
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Brenner DJ, et al. Ten new species of Legionella. Int. J. Syst. Bacteriol. 35: 50-59, 1985.