Flavobacterium branchiophilum Wakabayashi et al.
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2. Using a single tube of #1299 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth.
5. Incubate all tubes at 20oC for 6-10 days then plate the culture onto #1299 agar for another 6-10 days.
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von Graevenitz A. Revised nomenclature of Campylobacter laridis, Enterobacter intermedium, and "Flavobacterium branchiophila". Int. J. Syst. Bacteriol. 40: 211, 1990. PubMed: 2223613
Wakabayashi H, et al. Flavobacterium branchiophila sp. nov., a causative agent of bacterial gill disease of freshwater fishes. Int. J. Syst. Bacteriol. 39: 213-216, 1989.
Bernardet JF, et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Bacteriol. 46: 128-148, 1996.