Rhodococcus fascians (Tilford) Goodfellow
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 48 hours.
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Nesterenko OA, et al. Rhodococcus luteus nom. nov. and Rhodococcus maris nom. nov.. Int. J. Syst. Bacteriol. 32: 1-14, 1982.
Klatte S, et al. Rhodococcus luteus is a later subjective synonym of Rhodococcus fascians. Int. J. Syst. Bacteriol. 44: 627-630, 1994.
type strain of Rhodococcus luteus