Acidovorax konjaci (Goto) Willems et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 24-36 hours.
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Goto M. Pseudomonas pseudoalcaligenes subsp. konjaci subsp. nov., the causal agent of bacterial leaf blight of konjac (Amorphophalus knojac Koch.). Int. J. Syst. Bacteriol. 33: 539-545, 1983.
Willems A, et al. Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. Int. J. Syst. Bacteriol. 42: 107-119, 1992. PubMed: 1371056