Sphingobacterium spiritivorum (Holmes et al.) Yabuuchi et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
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Holmes B, et al. Flavobacterium spiritivorum, a new species isolated from human clinical specimens. Int. J. Syst. Bacteriol. 32: 157-165, 1982.
Yabuuchi E, et al. Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: Glucose-nonfermenting gram-negative rods in CDC groups IIK-2 and IIb. Int. J. Syst. Bacteriol. 33: 580-598, 1983.
Validation list no. 47. Int. J. Syst. Bacteriol. 43: 864-865, 1993.
Takeuchi M, Yokota A. Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov., Sphingobacterium thalpophilum comb. nov., and two genospecies of the genus Sphingobacterium, and synonymy of Falvobacterium yabuuchiae and Sphingobacterium spiritivorum. J. Gen. Appl. Microbiol. 38: 465-482, 1992.