Lipomyces tetrasporus Krasil'nikov et al. ex Nieuwdorp et al., teleomorph
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1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
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Nieuwdorp PJ, et al. Classification of Lipomyces. Antonie van Leeuwenhoek 40: 241-254, 1974. PubMed: 4599856
Kuek C. Production of glucoamylase using Aspergillus phoenicus immobilized in calcium alginate beads. Appl. Microbiol. Biotechnol. 35: 455-460, 1991.